Decondensation and Male Pronuclear Formation in Bovine Oocytes after Microinjection of Bovine Sperm Pretreated with Disulfide Bond Reducing Agents.
نویسندگان
چکیده
منابع مشابه
Progesterone treatment of boar spermatozoa improves male pronuclear formation after intracytoplasmic sperm injection into porcine oocytes.
Boar spermatozoa were prepared for intracytoplasmic sperm injection (ICSI) by two different treatments to facilitate sperm chromatin decondensation and improve fertilisation rates after ICSI in pigs: spermatozoa were either frozen and thawed without cryoprotectants, or treated with progesterone. Morphological changes of the sperm heads after the treatments were examined and then the activation ...
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Background Failure of Male Pronucleus (MPN) formation is a major concern in the success of Intracytoplasmic Sperm Injection (ICSI) in some species. Despite the conducted unsuccessful efforts to improve ICSI efficiency in ovine, the present study was aimed to improve MPN formation and embryo development in ovine ICSI procedure through accompaniment of sperm pretreatment with co-injection of some...
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Disulfide bonds tie different peptide chains together and lock folded dipeptide chains into particular configurations. It is of interest to learn whether there is a differential susceptibility of viruses to inactivation by disulfide bond reduction. This may lead to information concerning differences in virion structures and may help in grouping them in different morphological categories. Dithio...
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This study examined the event of protein phosphorylation in bovine oocytes during germinal vesicle breakdown (GVBD) and formation of pronuclei following fertilisation in vitro. Immature oocytes were obtained from abattoir materials and cultured in vitro. The oocytes were labelled with [32P]orthophosphate at 3 h intervals from 0 to 12 h following maturation in culture or from 3 to 18 h following...
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ژورنال
عنوان ژورنال: Journal of Mammalian Ova Research
سال: 1996
ISSN: 1341-7738,1347-5878
DOI: 10.1274/jmor.13.118